HPIC
About
High performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate, identify, and quantify various components of interest in a sample. This separation is accomplished through the instrument pumps moving pressurized liquid, what is referred to as the eluent, buffer, or liquid mobile phase, containing the sample liquid through a column packed with a solid adsorbent material. Next the separated components of the sample pass through the detectors which provide a chromatogram containing peaks unique to the sample components.
Ion chromatography separates the sample based on charge and is associated with our conductivity detector.
Located ES 508
Primary Contact
Alyssa Tsukada
she/her
tsukada2@wwu.edu
(360) 650-7577
ES 508d
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Agilent 1100 Series Stack
Primary Use:
Analysis of anions in water.
Detectors:
Waters 432 conductivity detector
Diode Array detector (DAD) / Multiple Wavelength UV-Vis detector
Refractive Index detector (RI)
Available Anion Standards:
Fluoride, Formate, Chloride, Nitrite, Bromide, Nitrate, Phosphate, and Sulfate.
Sample Preparation
Samples must be in liquid form and fully miscible with the method's running buffer. Prior to analysis, each sample should be filtered through a 0.2–0.45 µm filter into a 2 mL screw-top glass HPLC vial and securely capped with a 9 mm screw cap containing a rubber septum.
Recommended vials and cap examples below:
Alkali Scientific HPLC chromatography vial, with writing area, flat base with graduations, 9425 open top ribbed screw cap with 9 mm white PTFE/red silicone septa 1 mm thick, vial size: 12×32 mm, 100/pk
Part # 104027-086
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