Reverse-phase HPLC


High performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate, identify, and quantify various components of interest in a sample. This separation is accomplished through the instrument pumps moving pressurized liquid, what is referred to as the eluent, buffer, or liquid mobile phase, containing the sample liquid through a column packed with a solid adsorbent material. Next the separated components of the sample pass through the detectors which provide a chromatogram containing peaks unique to the sample components.

Reverse-phase chromatography separates the sample based on polarity and is associated with our multiple wavelength UV-Vis detector, refractive index detector, and fluorescence detectors.

Located ES 504

Primary Contact

Alyssa Tsukada 

(360) 650-7577
ES 508d

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Our Instrument

Primary Use:

Reverse-phase chromatography, but capable of a variety of chromatography types based on chosen attached column.


Fluorescence Detector

Diode Array detector (DAD) / Multiple Wavelength UV-Vis detector

Sample Preparation

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Educational Content

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Published Data

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