Reverse-phase HPLC
About
High performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate, identify, and quantify various components of interest in a sample. This separation is accomplished through the instrument pumps moving pressurized liquid, what is referred to as the eluent, buffer, or liquid mobile phase, containing the sample liquid through a column packed with a solid adsorbent material. Next the separated components of the sample pass through the detectors which provide a chromatogram containing peaks unique to the sample components.
Reverse-phase chromatography separates the sample based on polarity and is associated with our multiple wavelength UV-Vis detector, refractive index detector, and fluorescence detectors.
Located ES 504
Primary Contact
Alyssa Tsukada
she/her
tsukada2@wwu.edu
(360) 650-7577
ES 508d
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Our Instrument
Primary Use:
Reverse-phase chromatography, but capable of a variety of chromatography types based on chosen attached column.
Detectors:
Fluorescence Detector
Diode Array detector (DAD) / Multiple Wavelength UV-Vis detector
Sample Preparation
Samples must be in liquid form and fully miscible with the method's running buffer. Prior to analysis, each sample should be filtered through a 0.2–0.45 µm filter into a 2 mL screw-top glass HPLC vial and securely capped with a 9 mm screw cap containing a rubber septum.
Recommended vials and cap examples below:
Alkali Scientific HPLC chromatography vial, with writing area, flat base with graduations, 9425 open top ribbed screw cap with 9 mm white PTFE/red silicone septa 1 mm thick, vial size: 12×32 mm, 100/pk
Part # 104027-086
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